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DOI: 10.1530/eje.1.01811
European Journal of Endocrinology, Vol 152, Issue 1, 95-101
Copyright © 2005 by European Society of Endocrinology
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CLINICAL STUDY

Mechanism of inhibition of cytochrome P450 C21 enzyme activity by autoantibodies from patients with Addison’s disease

L Nikfarjam1, S Kominami1, T Yamazaki1, S Chen2, R Hewer2, C Dal Pra2,3, T Nakamatsu2,3, C Betterle4, R Zanchetta4, B Rees Smith2,3 and J Furmaniak2,3

1 Faculty of Integrated Arts and Sciences, Hiroshima University, Higashihiroshima, 739-8521 Japan, 2 FIRS Laboratories, RSR Ltd, Parc Ty Glas, Llanishen, Cardiff CF14 5DU, UK, 3 Department of Medicine, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK and 4 Department of Medical and Surgical Sciences, University of Padua, Via Ospedale Civile 105, 35128 Padua, Italy

(Correspondence should be addressed to J Furmaniak, FIRS Laboratories, RSR Ltd, Parc Ty Glas, Llanishen, Cardiff CF14 5DU, UK; Email: firs{at}rsrltd.eclipse.co.uk)


   Abstract
Top
 Abstract
Introduction
 Materials and methods
 Results
 Discussion
 References
 
Objective: To study possible mechanisms for the inhibition of cytochrome P450 C21 (steroid 21-hydroxylase) enzyme activity by P450 C21 autoantibodies (Abs) in vitro.

Design: Two possible mechanisms for the inhibition of P450 C21 enzyme activity by P450 C21 Abs were studied: (a) conformational changes in the P450 C21 molecule induced by Ab binding and (b) the effects of Ab binding to P450 C21 on the electron transfer from the nicotinamide adenine dinucleotide phosphate reduced (NADPH) cytochrome P450 reductase (CPR) to P450 C21.

Methods: The effect of P450 C21 Ab binding on the conformation of recombinant P450 C21 in yeast microsomes was studied using an analysis of the dithionite-reduced CO difference spectra. The effect of P450 C21 Abs on electron transfer was assessed by analysis of reduction of P450 C21 in the microsomes in the presence of CO after addition of NADPH.

Results: Our studies confirmed the inhibiting effect of P450 C21 Abs on P450 C21 enzyme activity. Binding of the Abs did not induce significant change in the P450 C21 peak at 450 nm (native form) and did not produce a detectable peak at 420 nm (denatured form) in the dithionite-reduced CO difference spectra. This indicated that conformation of P450 C21 around the heme was not altered compared with the native structure. However, incubation of the P450 C21 in yeast microsomes with P450 C21 Ab inhibited the fast phase electron transfer from the CPR to P450 C21.

Conclusions: Our observations suggested that the mechanism by which P450 C21 Abs inhibit P450 C21 enzyme activity most likely involves inhibition of the interaction between the CPR and P450 C21.


   Introduction
Top
 Abstract
 Introduction
Materials and methods
 Results
 Discussion
 References
 
Autoimmune Addison’s disease (AD) is characterised by the presence of autoantibodies (Abs) to steroid 21-hydroxylase (cytochrome P450 C21) in patients’ sera (1–3). P450 C21 is a heme containing membrane-bound enzyme that catalyses the conversion of progesterone and 17{alpha}-hydroxyprogesterone into deoxy-corticosterone (DOC) and 11-deoxycortisol respectively (4). Hydroxylation reactions require a molecular oxygen and electron transfers from nicotinamide adenine dinucleotide phosphate reduced (NADPH) via NADPH-cytochrome P450 reductase (CPR) (5). The heme binding site as well as the site for interaction with the CPR are situated in the C-terminal region of the P450 C21 molecule (6, 7). The correct tertiary structure of P450 C21 is important for P450 C21 enzyme activity (8, 9).

Previous studies have shown that P450 C21 Abs in sera from patients with AD have an inhibiting effect on P450 C21 enzyme activity in vitro (10) although this effect does not appear to be evident in vivo (11). P450 C21 Abs react with conformational epitopes located mostly in the central and the C-terminal part of the P450 C21 molecule (amino acids (aa) 280–494) and a close relationship between P450 C21 Abbinding sites and sites important for P450 C21 enzyme activity has been reported (3, 12–15).

We have studied two possible mechanisms for the inhibition of P450 C21 activity by P450 C21 Abs: (a) conformational changes in the P450 C21 molecule induced by the Ab binding and (b) the effects of the Abs binding to P450 C21 on the electron transfer from the CPR to P450 C21.


   Materials and methods
Top
 Abstract
 Introduction
 Materials and methods
Results
 Discussion
 References
 
P450 C21 Ab preparations

Sera from ten patients (P1–P10; Table 1Go) with auto-immune AD and P450 C21 Ab levels ranging from 16 units/ml to 2664 units/ml were used in the study. Five out of ten sera were from patients with isolated AD and five out of ten were from patients with autoimmune polyglandular syndrome (APS) type II (3). P450 C21 Abs were measured using an assay based on 125I-labelled recombinant P450 C21 (16) (kit from RSR Ltd, Cardiff, UK). IgG was isolated from the sera by chromatography on ProsepA (Millipore UK Ltd, Watford, Herts, UK) according to the manufacturer’s instructions, dialysed against phosphate-buffered saline (PBS; 8.1 mmol/l Na2HPO4, 1.5 mmol/l KH2PO4, 2.7 mmol/l KCl and 137 mmol/l NaCl, pH 7.3) and stored in aliquots at –70 °C. The IgG concentration was calculated on the basis of absorbance (Abs) at 280 nm of 1.0 = 0.70 mg/ml. In addition, IgGs were isolated from three P450 C21 Ab-negative healthy blood donor (HBD1-3) sera. The ability of IgGs isolated from AD sera and HBD sera to bind 125I-labelled P450 C21 was tested in the same way as serum P450 C21 Abs (16).


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  		Table 1 Characteristics of IgG samples used in the study and the effect on the fast phase reduction of P450 C21.
 
Patients gave informed consent for the study and HBD sera were purchased from Golden West Biologicals (Vista, CA, USA).

Recombinant human P450 C21 preparations

Full length recombinant human P450 C21was expressed in Saccharomyces cerevisiae as described previously (16, 18). Yeast transformed with P450 C21 cDNA was grown, harvested and broken as described previously (16, 18). The broken cells were centrifuged at 17 000 g for 30 min at 4 °C, and the supernatant was separated and then centrifuged at 105 000 g for 1 h at 4 °C. The microsomal pellet thus obtained was suspended in PBS followed by resedimentation at 105 000 g for 1 h at 4 °C and this cycle was repeated. The final pellet was resuspended in PBS (protein concentration of 10 mg/ml as determined by the Bradford assay) (19) and stored in aliquots at –70 °C.

Determination of P450 C21 enzyme activity

P450 C21 enzyme activity in the microsomal preparations was measured in terms of conversion of [3H]progesterone to [3H]DOC as described previously (5, 10, 20). IgGs from AD patients and HBDs were incubated with the microsomes in order to study the effect of IgGs on P450 C21 enzyme activity. Briefly, yeast microsomes containing recombinant P450 C21 (0.5 µg protein/1µl PBS) were incubated with IgG preparations (25 µg, 50 µg or 100 µg) in 50 µl PBS at 0 °C for 16 h. The CPR (9.4 pmol/0.7 µl in 50 mmol/l potassium phosphate buffer, pH 7.2 containing 20% (v/v) glycerol, 0.1 mmol/l EDTA and 0.1 mmol/l dithiothreitol), purified from bovine liver microsomes as described previously (20) was then added to the microsome–IgG mixture. After an incubation at 0 °C for 1 h, 5 nmol progesterone with 0.2 µCi [3H]progesterone (Perkin Elmer Life Sciences, Boston, MA, USA) in 500 µl of 50 mmol/l potassium phosphate buffer (pH = 7.2) containing 0.1 mmol/l EDTA was added and incubated at 37 °C for 1 min. The hydroxylation reaction was initiated by the addition of 10 µl of 10 mmol/l NADPH (Roche Diagnostics, Lewes, East Sussex, UK). After 40 min of incubation at 37 °C, the reaction was terminated by vigorous shaking with 1 ml chloroform containing 1.5 nCi [14C]DOC as an internal standard. [14C]DOC was prepared enzymatically from [14C]progesterone (Perkin Elmer Life Sciences) and purified by HPLC. [3H]DOC, the metabolite of [3H]progesterone, was separated by HPLC (JASCO PU-980 intelligent HPLC pump, Metrotech Services Ltd, Cork, Ireland; TSK UV-8 model-II, TSK TOYO, Soda, Japan; Tosoh AS-8000, TOSOH, Tokyo, Japan; Gilson 202, Gilson Inc., Middleton, IW, USA) using a normal phase silica-gel column (4.6 x 150 mm; Cosmosil 5SL; Nacalai Tesque, Kyoto, Japan) with hexane, isopropyl alcohol and acetic acid (in the ratio of 93:7:1 respectively) at a flow rate of 0.8 ml/min (20).

Measurement of CO difference spectra of P450 C21 preparations

Aliquots of 10 µg yeast microsomes (in 20 µl PBS) were preincubated with 420 µg IgG preparations (in 210 µl PBS) at 0 °C overnight and mixed with 480 µl reaction buffer (50 mmol/l potassium phosphate buffer, pH = 7.2, containing 0.1 mmol/l EDTA, 10 µmol/l progesterone and 50 mmol/l glucose). A few grains of dithionite (Nacalai Tesque) were added to the solution, mixed and incubated at room temperature for 5 min. The baseline spectrum of the reaction mixture was recorded (Beckman DU640s spectrophotometer, Beckman Coulter Inc., Fullerton, CA, USA) from 500 nm to 400 nm. Subsequently, CO gas was bubbled gently through the dithionite-reduced solution for a few seconds and the dithionite-CO difference spectra recorded at 25 °C (21). The difference spectra were obtained by subtraction of the baseline absorbance from the absorbance after dithionite reduction and CO bubbling.

Assessment of electron transfer from CPR to P450 C21

Aliquots containing 7.5 µg yeast microsome protein in 15 µl PBS were incubated with 280 µg IgG preparations (in 140 µl PBS) at 0 °C overnight. Then, 153 pmol CPR (9 µl) was added and incubated for 1 h at 0 °C. Reaction buffer (320 µl; see above) was degassed and bubbled with CO gas for a few seconds. This step was repeated three times. The mixture of the CPR, yeast microsomes and the IgG was then injected into the CO-saturated reaction buffer. The solution was further degassed and flushed with CO gas at least three times and the residual oxygen was removed by an oxygen scavenging system composed of 2 units glucose oxidase (from Aspergillus niger; Sigma-Aldrich Company Ltd, Poole, UK) and 60 units catalase (from bovine liver; Sigma-Aldrich Company Ltd, Poole, UK). After incubation at 25 °C for 5 min, the reaction was initiated by mixing the reaction mixture (502 µl) with 6 µl of 10 mmol/l NADPH in distilled water and the difference in the spectra of the reaction mixture before and after adding NADPH was recorded at 25 °C. The spectra were measured at 1, 2, 3, 5, 10, 20, 30, 40 and 55 min after NADPH addition. Subsequently, 2 µl saturated dithionite solution was added to the sample and the difference spectrum was recorded (complete reduction of P450 C21).

Statistical analysis

Statistical analyses of differences between the effect of patient and HBD IgGs on P450 C21 enzyme activity and the effect on electron transfer from CPR to P450 C21 were carried out using ANOVA in Origin version 6 (Rockware Inc., Golden, CO, USA). Values are given as means±S.D.


   Results
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 Abstract
 Introduction
 Materials and methods
 Results
Discussion
 References
 
The P450 C21 Ab activities of IgG preparations (P1–P10; Table 1Go) ranged from 13.4 to 1029 units/ml for 1 mg/ml solution of IgG when assessed using the [125I]P450 C21 assay (units are the kit calibrators). P450 C21 Ab activity was undetectable in IgG preparations from HBD sera.

P450 C21 enzyme activity in the microsomal preparation obtained from yeast transformed with P450 C21 cDNA, expressed as the ability to convert progesterone to DOC, was 1.22±0.25 (n = 12) nmol DOC produced/40 min per 0.5 µg microsomal protein. In contrast, DOC formation was undetectable (less than 0.02 nmol) in the absence of NADPH.

The effect of P450 C21 Ab on P450 C21 enzyme activity was expressed as the percentage of the activity remaining in the presence of patient IgG relative to the P450 C21 enzyme activity in the presence of HBD IgG. In the presence of 100 µg IgG from P1, P2 or P3, the remaining P450 C21 enzyme activity was 10±0.9%, 5±1.1% and 4.5±1.3% (closely agreeing duplicates) relative to the P450 C21 enzyme activity in the presence of HBD2 IgG respectively (Fig. 1Go). The differences in P450 C21 enzyme activity remaining in the presence of P1, P2 or P3 IgGs compared with HBD IgG were statistically significant (P < 0.001 in each case). In the case of experiments carried out with different concentrations of P3 IgG (25 µg, 50 µg or 100 µg), the remaining P450 C21 enzyme activity was 50±3%, 30±2.5% and 4.5±1.3% (closely agreeing duplicates), relative to the P450 C21 enzyme activity in the presence of the same concentrations of HBD2 IgG. P4–P10 IgGs (Table 1Go) were shown to have en effect on P450 C21 enzyme activity (2–63% of enzyme activity remaining) in our previous study (10).



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  		Figure 1 P450 C21 enzyme activity remaining in the presence of 100 µg IgGs from AD patients (P1, P2 or P3) relative to P450 C21 enzyme activity in the presence of 100 µg HBD2 IgG. Results shown are means of closely agreeing duplicates±S.D. The differences in P450 C21 enzyme activity remaining in the presence of P1, P2 or P3 IgGs compared with the activity remaining in the presence of HBD IgG were statistically significant (P < 0.001 in each case).

 
The conformation around the heme in the P450 molecule can be deduced from the dithionite-reduced CO difference spectra (21). The native form of P450 shows a peak at 450 nm in the difference spectra while the denatured form shows a peak at 420 nm. An analysis of dithionite-reduced CO difference spectra of P450 C21 in yeast microsomes in the presence of patient P2 and HBD3 are shown in Fig. 2Go. The difference spectra showed a peak at 450 nm in the presence of P2 or HBD3 IgGs (Fig. 2a and bGo respectively). The value of difference of absorbance at 450 nm minus absorbance at 490 nm ({Delta}OD) in the presence of HBD IgG was 0.005±0.0005 (n = 2) and was comparable with 0.0056±0.0005 (n=2) in the presence of P2 IgG. There was no peak evident at 420 nm in the difference spectra of the P450 C21 preparations incubated with P2 IgG or HBD3 IgG (Fig. 2Go). Similar results were obtained when P1, P3–P10 and HBD1 and HBD2 IgGs were tested (data not shown).



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  		Figure 2 Dithionate-reduced CO difference spectra of P450 C21 in the presence of (a) HBD3 IgG and (b) AD patient P2 IgG. The difference spectra show a peak at 450 nm but not at 420 nm in the presence of P2 or HBD3 IgGs.

 
Electron transfer from the CPR to P450 C21 heme can be detected by an increase of absorbance at 450 nm which is caused by immediate binding of CO to the reduced heme in P450 C21 (22). The increase of absorbance at 450 nm in the difference spectra of P450 C21 in the presence of HBD3 IgG after incubation with NADPH is shown in Fig. 3aGo. The absorbance at 450 nm increased rapidly to {Delta}OD Abs=0.0016 in 1 min after addition of NADPH and then increased slowly to about {Delta}OD=0.0055 at 55 min. In the case of P450 C21 preparations incubated with P2 IgG there was no peak at 450 nm at 1 min after incubation with NADPH (Fig. 3bGo). However, an increase of absorbance at 450 nm was observed over 2–55 min (max {Delta}OD of 0.0048) after addition of NADPH (Fig. 3bGo).



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  		Figure 3 CO difference spectra of P450 C21 in the presence of (a) HBD3 IgG and (b) AD patient P2 IgG. Absorbances represented by curves 1 to 4 were recorded after 1, 5, 20 and 55 min respectively after incubation with NADPH. Absorbances shown by curve 5 represent the spectrum after addition of dithionate (complete reduction of P450 C21).

 
The plot of an increase of absorbance at 450 nm over 55 min after addition of NADPH in the presence of HBD3 IgG or P2 IgG is shown in Fig. 4Go. As illustrated, the contribution of slow phase reduction within 1 min must be considered in calculating the fast phase reduction and consequently the true fast phase reduction within 1 min was obtained by subtracting the effect of slow phase reduction from the observed {Delta}OD at 1 min, i.e. [observed {Delta}OD in 1 min] – [(slope in the slow phase ({Delta}OD/min) x 1 min)] (Fig. 4Go). In the case of incubation of P450 C21 with HBD3 IgG, the value of true fast phase reduction was 0.0005 compared with 0.0001 in the case of incubation with P2 IgG.



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  		Figure 4 Plot of an increase in absorbance of P450 C21 at 450 nm over 55 min after addition of NADPH in the presence of HBD3 IgG ({diamondsuit}) or P2 IgG ({blacksquare}). The dotted line represents fast phase reduction within 1 min (see text for details).

 
The proportion of P450 C21 reduced in a rapid phase in the presence of P1–P10 or HBD1–3 IgGs expressed as a percentage of P450 C21 reduced with dithionite: [({Delta}OD in 1 min) – (slope in the slow phase ({Delta}OD/min)) x 1 min)]/[({Delta}OD after dithionite reduction]] is shown in Table 1Go.

There was a statistically significant difference in the percentage of P450 C21 reduced in the rapid phase in the case of incubation with nine out of ten IgGs from AD patients (P1–P9) compared with HBD IgG (P < 0.05 in each case). However, in the case of P10 IgG, the percentage of P450 C21 reduced in the rapid phase was not statistically different compared with HBD IgG (P = 0.86).


   Discussion
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 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
References
 
Incubation of P450 C21 preparations with P1–P3 IgGs resulted in an inhibition of P450 C21 enzyme activity (Fig. 1Go) and this is in agreement with the previously reported effect of P4–P10 IgGs (Table 1Go) (10). Furthermore, the degree of the inhibiting effect of P1–P3 IgGs on P450 C21 enzyme activity was similar (the remaining P450 C21 enzyme activity was 10, 5 and 4.5% in the case of incubation with P1, P2 and P3 IgGs containing 76.7 units/mg, 114.1 units/mg and 1029 units/mg P450 C21 Ab respectively (Table 1Go)). This is consistent with our previous observations that the degree of inhibition of P450 C21 enzyme activity was not related to the level of P450 C21 Abs in the IgG preparations (10). The aim of this study was to investigate the possible mechanism by which P450 C21 Abs inhibit P450 C21 enzyme activity. Correct conformational folding of the P450 C21 molecule and availability of an efficient system for electron transfer are assumed to be essential for P450 C21 enzyme activity (8, 9, 13–15, 22). Thus, there are at least two possible mechanisms by which P450 C21 Abs may inhibit P450 C21 enzyme activity: (a) changes in the conformation of the P450 C21 molecule brought about by binding of P450 C21 Abs and/or (b) interference in the electron transfer from the CPR to the P450 C21 heme as a result of P450 C21 Ab binding to P450 C21.

Analysis of dithionite-reduced CO difference spectra is a useful method to assess the conformation around the heme groups in P450 cytochrome proteins (21). The difference spectra of P450 proteins in their native conformation show a peak at 450 nm whereas in the case of denatured P450 proteins the highest absorbance is observed at 420 nm (21). We have studied the difference spectra of P450 C21 following incubation with IgGs isolated from P450 C21 Ab-positive AD patients or HBD. The difference spectra following incubation of P450 C21 with either P450 C21 Ab-positive or P450 C21 Ab-negative IgGs showed a peak at 450 nm. Furthermore, the peak height was similar after incubation with both types of IgGs (Fig. 2Go). There was no peak at 420 nm indicating that the conformation of P450 C21 around the heme molecule had not changed upon binding of P450 C21 Abs.

Electron transfer from NADPH-P450 reductase to P450 C21 can be measured by monitoring absorbance at 450 nm in the presence of CO (22). In the reaction, as soon as the P450 heme accepts an electron, CO binds to the heme and this is reflected by an increase of absorbtion at 450 nm. The physiological rate of electron transfer is fast; transfer of one electron from the CPR to P450 C21 in the P450 C21 enzyme activity reaction is estimated to take only a few seconds (22). Consequently, changes in the fast phase (within 1 min) of difference spectra at 450 nm are indicative of electron transfer related to P450 C21 enzyme activity (22). Figure 3aGo shows a rapid increase of a peak at 450 nm in the difference spectra within 1 min (curve 1) after addition of NADPH in the reaction with P450 C21 preparations preincubated with IgG from an HBD. However, there was no change of a peak at 450 nm (within 1 min after addition of NADPH) in the difference spectra in the case of the experiment carried out using P450 C21 preparations preincubated with P2 IgG (Fig. 3bGo, curve 1). These experiments allowed an assessment of the rate of the fast phase reduction of P450 C21 that was shown to be slower in the presence of nine out of ten patient IgGs compared with HBD IgG (Fig. 4Go and Table 1Go). The degree of the effect of P450 C21 Ab IgGs on the fast phase reduction of P450 C21 did not appear to be related to the levels of P450 C21 Ab in the IgG preparations or the form of AD (isolated AD or APS type II) (Table 1Go). The complexity of the experiments and the limited availability of patient IgGs did not allow us to extend the study with larger numbers of samples or samples from patients with APS type I or patients who were positive for P450 C21 Abs but had normal adrenal function. However, previous studies have shown that the binding characteristics of P450 C21 Ab in sera from patients with different forms of AD and P450 C21 Ab-positive patients without overt adrenal failure are similar and likely to recognise closely related epitopes on P450 C21 (3, 15, 17, 23). Furthermore, binding of all IgGs used in our study (P1–P10) to 125I-labelled P450 C21 was inhibited by a mixture of the three mouse MAb Fabs reactive with the epitopes within the C-terminal part of the P450 C21 molecule (aa 335–339, aa 391–405 and aa 406–411) (Table 1Go) (17). Mouse MAb Fabs inhibited most of the P450 C21 Ab activity in P1–P10 IgGs (81–93% inhibition; Table 1Go) indicating that the epitopes recognised by these Abs were closely related and located predominantly in the C-terminal part of P450 C21.

In our study, P10 IgG (only one out of ten patient IgGs studied) did not show a statistically significant effect on the fast phase P450 C21 reduction (Table 1Go). This IgG had a relatively small effect on P450 C21 enzyme activity (63% enzyme activity remaining in the presence of P10) (10) although the levels of P450 C21 Ab in this preparation were comparable with the levels in other preparations (Table 1Go).

The rate of slow phase electron transfer (reduction of P450 C21) was similar in the reactions with P450 C21 preparations in the presence of P450 C21 Abs or normal IgGs (Fig. 3aGo, curves 2–4; Fig. 3bGo, curves 2–4 and Fig. 4Go). Two step reduction of P450 cytochrome proteins in microsomal preparations or in the reconstituted systems (the mixture of purified P450 and the CPR) has been reported previously (22). However, it has been suggested that the slow rate of electron transfer is too slow to be effective for physiological catalytic activity of P450 C21 (22).

In this study we have not been able to detect conformational changes around the heme binding site in the P450 C21 molecule following binding of P450 C21 Abs. It is unlikely therefore that P450 C21 Abs inhibit P450 C21 enzyme activity through the conformational changes of P450 C21 brought about by P450 C21 Ab binding. However, our experiments do indicate that P450 C21 Abs in AD sera inhibit the fast phase of electron transfer from the CPR to P450 C21. Consequently, inhibition of electron transfer seems the most likely mechanism by which P450 C21 Abs inhibit P450 C21 enzyme activity. Alternatively, P450 C21 Ab binding may result in subtle conformational changes within the P450 C21 molecule that were not detected in the dithionite-reduced CO difference spectra experiments that we have carried out. It is not clear at present how P450 C21 Abs inhibit the electron transfer. One of the ways could be that P450 C21 Abs inhibit the interaction between the CPR and the P450 C21 molecule. For example, P450 C21 Ab-binding sites may be closely related to the CPR interaction site on P450 C21. Previous studies have shown the relationship between P450 C21 Ab-binding sites and sites important for P450 C21 enzyme activity (3, 10, 12–15). Furthermore, the P450 C21 Abs used in this study were directed predominantly to epitopes within the C-terminal part of P450 C21 known to be involved in interaction with the CPR (6, 7, 9, 24). However, the detailed location of P450 C21 epitopes involved in P450 C21 Ab binding has not been identified as yet (3, 12–15, 17, 23). It may well be that the lack of a clear effect on the fast phase reduction of P450 C21 by P10 in our study is related to the subtle differences in the binding sites for different P450 C21 Abs. Although our studies provide an insight into the mechanism of the inhibiting effect of P450 C21 Abs on P450 C21 enzyme activity in vitro, the significance of this inhibition for the pathogenesis of AD in vivo needs to be studied further (3). Also, more detailed analysis of the epitopes involved in P450 C21 Ab binding and their relationship to the CPR-binding site should be helpful in elucidating the mechanism by which P450 C21 Abs inhibit the fast phase electron transfer between the CPR and P450 C21.


   Acknowledgements
 
C D P and T N were in receipt of RSR fellowships. Carol James prepared the manuscript.


   References
Top
 Abstract
 Introduction
 Materials and methods
 Results
 Discussion
 References
 

    1. Baumann-Antczak A, Wedlock N, Bednarek J, Kiso Y, Krishnan H, Fowler S, Smith BR & Furmaniak J. Autoimmune Addison’s disease and 21-hydroxylase. Lancet 1992 340 429–430.

    2. Winqvist O, Karlsson FA & Kämpe O. 21-Hydroxylase, a major autoantigen in idiopathic Addison’s disease. Lancet 1992 339 1559–1562.[CrossRef][ISI][Medline]

    3. Betterle C, Dal Pra C, Mantero F & Zanchetta R. Autoimmune adrenal insufficiency and autoimmune polyendocrine syndromes: autoantibodies, autoantigens, and their applicability in diagnosis and disease prediction. Endocrine Reviews 2002 23 327–364.[Abstract/Free Full Text]

    4. Miller WL. Genetics, diagnosis and management of 21-hydroxylase deficiency. Journal of Clinical Endocrinology and Metabolism 1994 78 241–246.[CrossRef][ISI][Medline]

    5. Takemori S & Kominami S. Steroidogenic electron transfer system of adrenal cortex microsomes. In Oxygenases and Oxygen Metabolism, pp 403–408. Ed. M Nozaki. Tokyo: Academic Press, 1982.

    6. Chiou S-H, Hu M-C & Chung B-C. A missense mutation at Ile172–Asn or Arg356–Trp causes steroid 21-hydroxylase deficiency. Journal of Biological Chemistry 1990 265 3549–3552.[Abstract/Free Full Text]

    7. Yoshioka H, Morohashi K, Sogawa K, Yamane M, Kominami S, Takemori S, Okada Y, Omura T & Fujii-Kuriyama Y. Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex. Journal of Biological Chemistry 1986 261 4106–4109.[Abstract/Free Full Text]

    8. White PC & Speiser PW. Congenital adrenal hyperplasia due to 21-hydroxylase deficiency. Endocrine Reviews 2000 21 245–291.[Abstract/Free Full Text]

    9. Mornet E & Gibrat J-F. A 3D model of human P450C21: study of the putative effects of steroid 21-hydroxylase gene mutations. Human Genetics 2000 106 330–339.[CrossRef][ISI][Medline]

    10. Furmaniak J, Kominami S, Asawa T, Wedlock N, Colls J & Rees Smith B. Autoimmune Addison’s disease – evidence for a role of steroid 21-hydroxylase autoantibodies in adrenal insufficiency. Journal of Clinical Endocrinology and Metabolism 1994 79 1517–1521.[Abstract]

    11. Boscaro M, Betterle C, Volpato M, Fallo F, Furmaniak J, Rees Smith B & Sonino N. Hormonal responses during various phases of autoimmune adrenal failure: no evidence for 21-hydroxylase enzyme activity inhibition in vivo. Journal of Clinical Endocrinology and Metabolism 1996 81 2801–2804.[Abstract]

    12. Wedlock N, Asawa T, Baumann-Antczak A, Rees Smith B & Furmaniak J. Autoimmune Addison’s disease. Analysis of autoantibody binding sites on human steroid 21-hydroxylase. FEBS Letters 1993 332 123–126.[CrossRef][ISI][Medline]

    13. Asawa T, Wedlock N, Baumann-Antczak A, Rees Smith B & Furmaniak J. Naturally occurring mutations in human steroid 21-hydroxylase influence adrenal autoantibody binding. Journal of Clinical Endocrinology and Metabolism 1994 79 372–376.[Abstract]

    14. Tanaka H, Asawa T, Powell M, Chen S, Rees Smith B & Furmaniak J. Autoantibody binding to steroid 21-hydroxylase – effect of five mutations. Autoimmunity 1997 26 253–259.[ISI][Medline]

    15. Nikoshkov A, Falorni A, Lajic S, Laureti S, Wedell A, Lernmark K & Luthman H. A conformational-dependent epitope in Addison’s disease and other endocrinological autoimmune diseases maps to a carboxyl-terminal function domain of human steroid 21-hydroxylase. Journal of Autoimmunity 1999 162 2422–2426.

    16. Tanaka H, Perez MS, Powell M, Sanders JF, Sawicka J, Chen S, Prentice L, Asawa T, Betterle C, Volpato M, Smith BR & Furmaniak J. Steroid 21-hydroxylase autoantibodies: measurements with a new immunoprecipitation assay. Journal of Clinical Endocrinology and Metabolism 1997 82 1440–1446.[Abstract/Free Full Text]

    17. Chen S, Sawicka J, Prentice L, Sanders JF, Tanaka H, Petersen V, Betterle C, Volpato M, Roberts S, Powell M, Smith BR & Furmaniak J. Analysis of autoantibody epitopes on steroid 21-hydroxylase using a panel of monoclonal antibodies. Journal of Clinical Endocrinology and Metabolism 1998 83 2977–2986.[Abstract/Free Full Text]

    18. Bednarek J, Furmaniak J, Wedlock N, Kiso Y, Baumann-Antczak A, Fowler S, Krishnan H, Craft JA & Rees Smith B. Steroid 21-hydroxylase is a major autoantigen involved in adult onset autoimmune Addison’s disease. FEBS Letters 1992 309 51–55.[CrossRef][Medline]

    19. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976 72 248–254.[CrossRef][ISI][Medline]

    20. Higuchi A, Kominami S & Takemori S. Kinetic control of steroidogenesis by steroid concentration in guinea pig adrenal microsomes. Biochimica et Biophysica Acta 1991 1084 240–246.[Medline]

    21. Omura T & Sato R. The carbon monoxide binding pigment of liver microsomes. I – evidence for its hemoprotein nature. Journal of Biological Chemistry 1964 239 2370–2378.[Free Full Text]

    22. Kominami S, Hara H, Ogishima T & Takemori S. Interaction between cytochrome P450 (P450 C21) and NADPH-cytochrome P450 reductase from adrenocortical microsomes in a reconstituted system. Journal of Biological Chemistry 1984 259 2991–2999.[Abstract/Free Full Text]

    23. Volpato M, Prentice L, Chen S, Betterle C, Rees Smith B & Furmaniak J. A study of the epitopes on steroid 21-hydroxylase recognized by autoantibodies in patients with or without Addison’s disease. Clinical and Experimental Immunology 1998 111 422–428.[CrossRef][Medline]

    24. Lajic S, Levo A, Nikoshkov A, Lundberg Y, Partanen J & Wedell A. A cluster of missense mutations at Arg356 of human steroid 21-hydroxylase may impair redox partner interaction. Human Genetics 1997 99 704–709.[CrossRef][ISI][Medline]

Received 5 April 2004
Accepted 23 September 2004




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R. N. Miguel, S. Chen, L. Nikfarjam, S. Kominami, B. Carpenter, C. Dal Pra, C. Betterle, R. Zanchetta, T. Nakamatsu, M. Powell, et al.
Analysis of the interaction between human steroid 21-hydroxylase and various monoclonal antibodies using comparative structural modelling
Eur. J. Endocrinol., December 1, 2005; 153(6): 949 - 961.
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