Eur J Endocrinol
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DOI: 10.1530/EJE-08-0727
European Journal of Endocrinology, Vol 160, Issue 4, 585-592
Copyright © 2009 by European Society of Endocrinology
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CLINICAL STUDY

Secretion of adiponectin multimeric complexes from adipose tissue explants is not modified by very low calorie diet

Zuzana Kovacova1,2,4, Michaela Vitkova1,4, Michaela Kovacikova1,2,4, Eva Klimcakova1,4, Magda Bajzova1,3,4, Zuzana Hnevkovska1,3,4, Lenka Rossmeislova1,4,5, Vladimir Stich1,4, Dominique Langin4,5,6,7 and Jan Polak1,3

1 Sports Medicine Department2 Division of Cell and Molecular Biology, Third Faculty of Medicine, Charles University in Prague, Ruská 87, 100 00 Prague 10, Czech Republic3 2nd Internal Medicine Department, Vinohrady Teaching Hospital, 100 00 Prague, Czech Republic4 Franco Czech Laboratory for the Clinical Research on Obesity, INSERM France and Third Faculty of Medicine, Charles University, 100 00 Prague, Czech Republic5 Inserm, U858; Laboratoire de recherche sur les obésités, F-31432 Toulouse, France6 Université de Toulouse; UPS; Institut de Médecine Moléculaire de Rangueil; IFR31; F-31432 Toulouse, France7 CHU de Toulouse, Laboratoire de Biochmie, IFB, F-31000 Toulouse, France

(Correspondence should be addressed to Z Kovacova; Email: zuzana.kovacova{at}post.lf3.cuni.cz)

Objective: Adiponectin is a protein abundantly secreted by the adipose tissue (AT). Plasma adiponectin levels are decreased in obese, insulin-resistant, and type 2 diabetic patients. Various multimeric complexes, i.e. high-, middle-, and low-molecular weight isoforms (HMW, MMW and LMW), are present in plasma. Here, we investigated the effect of weight reducing diet on the distribution of adiponectin isoforms in plasma and on their secretion in AT explants from obese subjects.

Design: A total of 20 obese subjects (age 37.8±7.3 years, body mass index 33.9±5.0 kg/m2) underwent eight weeks of very low-calorie diet (VLCD). A needle biopsy of subcutaneous abdominal AT and blood samples were taken before and after dietary intervention. AT explants were incubated in culture medium for 4 h. ELISA assay and western blot analyses were used to identify adiponectin complexes in culture media and in plasma.

Results: The distribution of adiponectin polymers in plasma was different from that secreted in human AT explants. Before VLCD, the relative amount of HMW isoform was 75.5±9.1% of total adiponectin in culture media and 52.2±11.2% in plasma. Despite the diet-induced weight loss and improvement of insulin sensitivity, VLCD neither induced change in total adiponectin level nor in the ratio of HMW to total adiponectin in plasma and in culture media of AT explants.

Conclusions: The profile of adiponectin polymeric isoforms secreted by AT explants into culture media differs from the plasma profile. A dietary intervention leading to weight loss and improvement of insulin sensitivity was not associated with modifications of AT secretion of total or HMW adiponectin.






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