Decreased plasma adiponectin concentrations in nondiabetic women with elevated homeostasis model assessment ratios

Division of Endocrinology and Metabolism, Otaru City General Hospital, Japan. miyamatsuba@proof.ocn.ne.jp

OBJECTIVE: Whether the adipocyte-derived protein adiponectin is associated with insulin resistance independently of the effects of adiposity and the diabetic state is an important question. We explored, in a cross-sectional study of 486 Japanese nondiabetic women, the relationship between the calculated insulin resistance (homeostasis model assessment ratio (HOMA-R)) and adiponectin levels determined using a validated sandwich ELISA. DESIGN AND METHODS: All participants were stratified into tertiles for HOMA-R (approximately <1.5, 1.5< or = approximately <3.0, 3.0< or = approximately ) and the differences across tertiles of continuous variables were tested with ANOVA. Two-way ANOVA was used to determine possible relationships for plasma adiponectin between tertiles of HOMA-R and several stratified parameters. Multiple regression analyses were performed with HOMA-R or fasting serum insulin as dependent variable, and diastolic blood pressure (BP), body mass index (BMI), serum triglyceride (TG), leptin and adiponectin as independent determinants. RESULTS: Mean plasma adiponectin in the high HOMA-R group decreased compared with that in the low HOMA-R group both before (mean+/-s.e.m. 6.2+/-0.6 vs 9.2+/-0.3 microg/ml, P<0.001) and after adjustment for body fat mass (BFM) as kg or percent (0.31+/-0.04 vs 0.69+/-0.03, 0.18+/-0.02 vs 0.34+/-0.01, both P<0.001). HOMA-R was inversely associated with adiponectin levels both before (r=-0.37, P<0.001) and after adjustment for BFM (r=-0.49, -0.46, both P<0.001). After covariate adjustment for age, diastolic BP, BMI and serum TG, HOMA-R retained a significant correlation with adiponectin/BFM (kg). Both adiponectin and leptin were the significant determinants of HOMA-R or fasting insulin in multiple regression models. CONCLUSIONS: Adiponectin was inversely associated with insulin resistance in nondiabetic subjects, independently from age, BP, adiposity and serum lipids. Because adiponectin is thought to have an anti-atherogenic action, the presence of hypoadiponectinemia may predispose subjects to atherosclerosis, and may progress the atherogenesis in insulin resistance.

This article has been cited by other articles:

				J Polak, Z Kovacova, C Holst, C Verdich, A Astrup, E Blaak, K Patel, J M Oppert, D Langin, J A Martinez, et al. Total adiponectin and adiponectin multimeric complexes in relation to weight loss-induced improvements in insulin sensitivity in obese women: the NUGENOB study. Eur. J. Endocrinol., April 1, 2008; 158(4): 533 - 541. [Abstract] [Full Text] [PDF]

				A Klamer, K Skogstrand, D M Hougaard, B Norgaard-Petersen, A Juul, and G Greisen Adiponectin levels measured in dried blood spot samples from neonates born small and appropriate for gestational age Eur. J. Endocrinol., August 1, 2007; 157(2): 189 - 194. [Abstract] [Full Text] [PDF]

				M. Bluher, A. M. Brennan, T. Kelesidis, J. Kratzsch, M. Fasshauer, S. Kralisch, C. J. Williams, and C. S. Mantzoros Total and High-Molecular Weight Adiponectin in Relation to Metabolic Variables at Baseline and in Response to an Exercise Treatment Program: Comparative evaluation of three assays Diabetes Care, February 1, 2007; 30(2): 280 - 285. [Abstract] [Full Text] [PDF]

				F. Conus, D. B. Allison, R. Rabasa-Lhoret, M. St-Onge, D. H. St-Pierre, A. Tremblay-Lebeau, and E. T. Poehlman Metabolic and Behavioral Characteristics of Metabolically Obese but Normal-Weight Women J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5013 - 5020. [Abstract] [Full Text] [PDF]

				E. Zoico, V. Di Francesco, G. Mazzali, R. Vettor, F. Fantin, L. Bissoli, S. Guariento, O. Bosello, and M. Zamboni Adipocytokines, Fat Distribution, and Insulin Resistance in Elderly Men and Women J. Gerontol. A Biol. Sci. Med. Sci., September 1, 2004; 59(9): M935 - M939. [Abstract] [Full Text] [PDF]

				B. Stiles, Y. Wang, A. Stahl, S. Bassilian, W. P. Lee, Y.-J. Kim, R. Sherwin, S. Devaskar, R. Lesche, M. A. Magnuson, et al. Liver-specific deletion of negative regulator Pten results in fatty liver and insulin hypersensitivity PNAS, February 17, 2004; 101(7): 2082 - 2087. [Abstract] [Full Text] [PDF]

				P. J. Havel Update on Adipocyte Hormones: Regulation of Energy Balance and Carbohydrate/Lipid Metabolism Diabetes, February 1, 2004; 53(90001): S143 - 151. [Abstract] [Full Text]