Eur J Endocrinol
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DOI: 10.1530/eje.0.1420506
European Journal of Endocrinology, Vol 142, Issue 5, 506-511
Copyright © 2000 by European Society of Endocrinology
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Articles

Effect of activin on production and secretion of prolactin and growth hormone in cultured rat GH3 cells

N Tamura, M Irahara, A Kuwahara, K Ushigoe, H Sugino, and T Aono

Department of Obstetrics and Gynecology, School of Medicine, University of Tokushima, Tokushima, 770-8503 Japan. noriko@shikoku.ne.jp

OBJECTIVE: To evaluate the effect of the growth factor activin A on the secretion of prolactin (PRL) and GH in cultured GH3 cells. METHODS: The concentrations of PRL and GH secreted from GH3 cells cultured in media with and without activin A were measured by RIA, and the expression of PRL mRNA and GH mRNA were analyzed using the Northern blot method. RESULTS: Activin A significantly inhibited PRL release from GH3 cells cultured for 48h in a dose-dependent manner (activin: 0.3-3nM). The inhibitory effects of 3nM activin A were observed in the culture from 12h to 48h (53.2% of control). Activin A (3nM) also significantly inhibited the expression of PRL mRNA at 24h (33.8% of control). In contrast, activin A significantly stimulated GH release from GH3 cells cultured for 48h in a dose-dependent manner (activin: 0.3-3nM). The stimulatory effect of 3nM activin A was observed in the culture for 48h (157.6% of control). Activin A (3nM) also significantly stimulated the expression of GH mRNA at 24h (183.6% of control). In spite of these significant changes in PRL and GH secretion, pit-1 mRNA levels were not significantly changed by activin A. CONCLUSIONS: These findings indicated that activin A modulates PRL and GH secretion through the regulation of PRL and GH gene transcription in GH3 cells, but that these effects are unrelated to pit-1 gene expression.


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