Eur J Endocrinol
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DOI: 10.1530/eje.0.1410585
European Journal of Endocrinology, Vol 141, Issue 6, 585-589
Copyright © 1999 by European Society of Endocrinology
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Articles

The phosphorylation status of insulin-like growth factor-binding protein-1 in prepubertal obese children

T Kamoda, H Saitoh, S Nakahara, M Inudoh, T Hirano, and A Matsui

Department of Pediatrics, University of Tsukuba, Tsukuba, Japan. tkamoda@md.tsukuba.ac.jp

OBJECTIVE: We measured the total and nonphosphorylated insulin-like growth factor-binding protein (IGFBP)-1 concentrations in obese children to determine the effect of obesity on the status of IGFBP-1 phosphorylation. We also measured the serum levels of insulin, total and free IGF-I, and IGFBP-3 to investigate their relationships to the IGFBP-1 phosphorylation status in obese subjects. SUBJECTS AND METHODS: Nineteen prepubertal obese and 15 age-matched control children were included in the study. The serum levels of total and nonphosphorylated IGFBP-1 were determined by noncompetitive RIAs. RESULTS: The serum levels of total and nonphosphorylated IGFBP-1 were significantly lower in the obese group (48.7+/-5.6 microgram/l, P<0.001 and 11.1+/-1.9 microgram/l, P<0.01 respectively) than in the controls (86.7+/-9.0 microgram/l and 28.8+/-6.2 microgram/l respectively). However, the ratio of nonphosphorylated IGFBP-1 to total IGFBP-1 did not differ significantly between the obese and control groups. The circulating free IGF-I level was significantly higher in the obese children than in the controls (P<0.05), while the serum levels of insulin, total IGF-I and IGFBP-3 were not significantly different between the two groups. A stepwise regression analysis of the combined group revealed that only the total IGFBP-1 level was an independent predictor of the free IGF-I concentration (P<0.001). CONCLUSION: The present study shows that both total and nonphosphorylated IGFBP-1 concentrations are decreased in obese children and the increased free IGF-I level in obese children is related to the reduced total IGFBP-1 level, but unrelated to the change in the IGFBP-1 phosphorylation status.


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E. Kajantie, L. Dunkel, E.-M. Rutanen, M. Seppala, R. Koistinen, A. Sarnesto, and S. Andersson
IGF-I, IGF Binding Protein (IGFBP)-3, Phosphoisoforms of IGFBP-1, and Postnatal Growth in Very Low Birth Weight Infants
J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2171 - 2179.
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