Eur J Endocrinol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

DOI: 10.1530/eje.0.1360223
European Journal of Endocrinology, Vol 136, Issue 2, 223-229
Copyright © 1997 by European Society of Endocrinology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheikh, R. B.
Right arrow Articles by Dumas, P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Cheikh, R. B.
Right arrow Articles by Dumas, P.

Influence of prolonged fasting and thyroid hormone on the {alpha}2-adrenergic response in isolated epididymal adipocytes of Wistar rats

Rajae Ben Cheikh, Philippe Chomard, Jean Luc Beltramo, Nicole Autissier and Paul Dumas

Fasting and thyroid hormone have been reported to modulate the β-adrenergic pathway of lipolysis in rat, but their effects on the {alpha}2-adrenergic response are not well known. The purpose of the present study was to investigate this point. Male Wistar rats, 3 weeks old, were thyroidectomized surgically, kept for 1 month at 25 °C and then fasted or not fasted for 3 days with or without daily intraperitoneal injection of 3,5,3'-tri-iodo-L-thyroni(T3; 4·6 nmol/100 g body weight). Age-matched, sham-operated, fed and fasted euthyroid rats were used as controls. The experiments were carried out using isolated epididymal adipocytes. The {alpha}2-adrenergic agonist UK 14304 (UK) inhibited the stimulated lipolysis more in fed than in fasted euthyroid rats whereas it had no effect in hypothyroid or T3-treated hypothyroid rats. The {alpha}2-adrenergic antagonist idazoxan reversed the antilipolytic effect of UK more in fasted than in fed euthyroid rats. The {alpha}2-adrenergic antagonist RX 82 1002 (RX) did so, but at lower concentrations than those of idazoxan. Idazoxan slightly increased the glycerol release in hypothyroid and especialiy T3-treated hypothyroid rats. RX had practically no effect on the production of glycerol in these animals. The findings suggest that (a) fasting and probably hypothyroidism decrease the {alpha}2-adrenergic response in adipocytes from rats, (b) T3 treatment of hypothyroid rats has no effect on the {alpha}2 response, and (c) thyroid hormone does not directly modulate the {alpha}2-adrenergic response in rat adipocytes.

European Journal of Endocrinology 136 223–229






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 European Society of Endocrinology.