Eur J Endocrinol
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DOI: 10.1530/eje.0.1330342
European Journal of Endocrinology, Vol 133, Issue 3, 342-346
Copyright © 1995 by European Society of Endocrinology
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Regulation of rat placental lactogen (rPL)-II secretion: cAMP inhibits rPL-II secretion in vitro

Masaaki Yamaguchi, Motoyuki Kawai, Kurajiro Kishi and Akira Miyake

Yamaguchi M, Kawai M, Kishi K, Miyake A, Regulation of rat placental lactogen (rPL)-II secretion: cAMP inhibits rPL-II secretion in vitro. Eur J Endocrinol 1995;133:342–6. ISSN 0804–4643

We examined whether epidermal growth factor (EGF), interleukin (IL)-1, IL-6 and cAMP analogs, which regulate mouse placental lactogen II secretion, affect rat placental lactogen (rPL)-II secretion using a rat choriocarcinoma cell line, Rcho-1. EGF, IL-1 and IL-6 did not affect rPL-II secretion, but 8-bromo-cAMP and forskolin inhibited rPL-II secretion by the 8th day of culture. The effects were dose dependent and the lowest concentrations of 8-bromo-cAMP and forskolin that significantly inhibited rPL-II secretion were 125 and 5 µmol/l, respectively. Cholera toxin also inhibited rPL-II secretion. The reverse hemolytic plaque assay for rPL-II indicated that the cells releasing rPL-II in the culture were giant cells and that 8-bromo-cAMP decreased the number of rPL-II-releasing cells. Western blot analysis of rPL-II yielded a single band at approximately 24.5 K, and 8-bromo-cAMP treatment significantly reduced the band intensity. Northern blot analysis of rPL-II indicated that 8-bromo-cAMP also reduced rPL-II gene expression. These findings suggest that the increase of intracellular cAMP accumulation results in inhibition of rPL-II secretion by decreasing rPL-II gene expression and inhibiting giant cell differentiation.

Masaaki Yamaguchi, Department of Obstetrics and Gynecology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, Japan






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