Eur J Endocrinol
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DOI: 10.1530/eje.0.1320681
European Journal of Endocrinology, Vol 132, Issue 6, 681-687
Copyright © 1995 by European Society of Endocrinology
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Urinary excretion rate of ceruloplasmin in non-insulin-dependent diabetic patients with different stages of nephropathy

Masatoshi Yamazaki, Seiki Ito, Akio Usami, Nagayuki Tani, Osamu Hanyu, Osamu Nakagawa, Hiroshi Nakamura and Akira Shibata

Yamazaki M, Ito S, Usami A, Tani N, Hanyu 0, Nakagawa O. Nakamura H, Shibata A. Urinary excretion rate of ceruloplasmin in non-insulin-dependent diabetic patients with different stages of nephropathy. Eur J Endocrinol 1995;132:681–7. ISSN 0804–4643

The level of ceruloplasmin, which is a more negatively charged protein than albumin, was measured by an immunoradiometric assay in timed overnight urine and serum samples from patients with non-insulin-dependent diabetes mellitus and healthy controls. None of the plasma proteins examined showed any cross-reactivity in this assay. A linear correlation was seen between the ceruloplasmin level and the serial dilution of the sample. Western blot analysis using concentrated urine samples showed that the molecular weight of ceruloplasmin in the urine sample was the same as that of ceruloplasmin in the serum and standard samples. These findings indicated that the substance detected by this assay was truly ceruloplasmin. The urinary ceruloplasmin excretion rate (CER) and clearance of ceruloplasmin increased in parallel with the progression of albuminuria. The highest CER was found in macroalbuminuric patients, followed by micro- and normoalbuminuric patients and the healthy control subjects, the differences between the groups being significant. In view of the fact that the isoelectric point of ceruloplasmin (4.4) is more acidic than that of albumin, the present findings suggested that an enhanced CER was due either to the alteration of charge selectivity in the glomerular basement membrane with unaltered tubular function or to a defect of the non-discriminatory pores (shunt pathway) with unaltered tubular function.

Seiki Ito, Division of Gerontology, Akita University Hospital, 1-1-1 Hondou, Akita City, Japan 010




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T. Narita, M. Hosoba, M. Kakei, and S. Ito
Increased Urinary Excretions of Immunoglobulin G, Ceruloplasmin, and Transferrin Predict Development of Microalbuminuria in Patients With Type 2 Diabetes
Diabetes Care, January 1, 2006; 29(1): 142 - 144.
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T. Narita, H. Sasaki, M. Hosoba, T. Miura, N. Yoshioka, T. Morii, T. Shimotomai, J. Koshimura, H. Fujita, M. Kakei, et al.
Parallel Increase in Urinary Excretion Rates of Immunoglobulin G, Ceruloplasmin, Transferrin, and Orosomucoid in Normoalbuminuric Type 2 Diabetic Patients
Diabetes Care, May 1, 2004; 27(5): 1176 - 1181.
[Abstract] [Full Text] [PDF]


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