HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by De Pergola, G. Articles by Björntorp, P. Search for Related Content PubMed Articles by De Pergola, G. Articles by Björntorp, P.

Estradiol regulation of mRNA expression of stimulatory G-protein -subunit in white adipose tissue from female rats

De Pergola G, Xu X, Carlsson B, Eriksson P, Edén S, Giorgino R, Björntorp P. Estradiol regulation of mRNA expression of stimulatory -subunit in white adipose tissue from female rats. Eur J Endocrinol 1994;130:146–50. ISSN 0804–4643

Adipose tissue has been recognized as a major peripheral metabolic target of estrogens. The present study was addressed to examine in female rats whether differences in the adipose tissue mRNA expression of -subunit of stimulatory (G_s) and/or inhibitory (G_j) G-proteins exist between intact and ovariectomized rats, the latter with or without estradiol or testosterone treatment. The fat cell membrane protein amount of G_s and G_j -subunit also was examined. All these parameters were evaluated in parametrial fat tissue samples obtained from 40 female Sprague-Dawley rats. A group of rats (N=20) was investigated for evaluation of mRNA expression and another group (N=20) for quantification of the protein amount of G_s and G_j -subunit. Each group was represented by five control rats (sham-operated), five ovariectomized (OVX) rats, five ovariectomized rats treated with estradiol (OVXE) and five ovariectomized rats treated with testosterone (OVXT). Ribonucleic acid extracted from adipose tissue and analyzed by northern blot with G_s, G^i-1 and G_i-2 cRNA probes revealed three major bands with estimated sizes of 1.9, 3.5 and 2.35 kb, respectively. Messenger RNA quantitative analysis, by a solution of hybridization RNAase protection assay on total nucleic acid samples, showed that the amount of G_i-1 and G_i-2 mRNA was similar within the different groups, whereas the G_s mRNA was significantly less abundant (p <0.01) in OVX and OVXT rats than in control or OVXE rats. No difference in G_s mRNA content was found between control and OVXE rats. As with mRNA analysis, protein quantitative analysis by an enzyme-linked immunosorbent assay in fat cell membrane preparation showed that the amount of G_i(1–2) was similar within the different groups and that the G_s content was significantly higher in control and OVXE rats than in OVX and OVXT rats (p<0.01). This study in female rats shows that estradiol may be involved in the control of protein amount and mRNA expression of G_s -subunit in adipose tissue. These results may explain in part how estrogens regulate the variations of body fat mass in female rats.

Giovanni De Pergola, via Putignani 236, 70122 Bari, Italy

This article has been cited by other articles:

				A. J. Morris and C. C. Malbon Physiological Regulation of G Protein-Linked Signaling Physiol Rev, October 1, 1999; 79(4): 1373 - 1430. [Abstract] [Full Text] [PDF]

				C. Darimont, R. Delansorne, J. Paris, G. Ailhaud, and R. Negrel Influence of Estrogenic Status on the Lipolytic Activity of Parametrial Adipose Tissue in Vivo: an in Situ Microdialysis Study Endocrinology, March 1, 1997; 138(3): 1092 - 1096. [Abstract] [Full Text] [PDF]